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You are here:Home » Molecular Biology » MB-CD Markers » CD14, Recombinant, Human (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)

CD14, Recombinant, Human (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific
Leucine-rich Glycoprotein)

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Specifications

CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocytes, macro¬pha¬ges and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50kD protein.
Catalog #C2265-37
SourceRecombinant corresponding to human CD-14, expressed in CHO cells.
Molecular Weight~50kD
Biological ActivityInhibits binding of LPS to CD14. Upto to 20ug/ml CD14 inhibit binding of FITC-LPS (0.5ug/ml) to 6x10e5 CD14+CHO transfectants (FACS)
Endotoxin <0.01ng/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~ 50kD
SourceCHO cells
Purity~90-95% (SDS-PAGE)
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 10ul ultra-pure dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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