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You are here:Home » Antibodies » Antibodies-Enzymes, Glycosyltransferase » Anti -CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)

Anti -CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT,
FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV,
Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Human CD15 is expressed by neutrophils, eosinophils and monocytes. Antibodies in this cluster recognize a common terminal pentasaccharide found on cell surface glycoproteins and glycolipids.
Catalog #C2266-01T
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 5ug/ml labels 5x10e5 cultured U-937 human tumor cells
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Five x10e5 cultured U-937 human tumor cells were incubated 45 minutes on ice with 80ul of C2266-01T at 5ug/ml.
2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.47 log10 fluorescent units when compared to a mouse IgM negative control at a similar concentration.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone NoAHN1.1
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride. No preservative added.
PurityPurified by Protein A affinity chromatography
ImmunogenHuman neutrophils
SpecificityRecognizes human CD15. Reacts with the human terminal pentasaccharide lacto-N-fucopentaose III known as Lewis X. Activates normal monocytes and inhibits neutrophil chemotaxis.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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