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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD16 (Fc gamma RIII alpha, NK cells)

Anti -CD16 (Fc gamma RIII alpha, NK cells)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IH FC IF
CD16 is the low affinity Fc gamma III present on Large Granular Lymphocytes (LGL), NK cells, granulocytes and a macrophage subpopulation. It is a late myeloid differentiation antigen and expressed on Chronic Myeloid Leukemia cells. The antibodies LGL/1 and LGL/3 show a polymorphism for granulocytes. LGL/1 reacts with granulocytes of a proportion of the human population and LGL/3 reacts with the granulocytes of another proportion of the population. The nature of this polymorphism of granulocytes has not yet been revealed.
Catalog #C2267-32
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5B11
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes CD16 antigen. Species Crossreactivity: human, rat and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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