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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2)

Anti -CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation.
Catalog #C2269
HybridomaFormed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse.
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Adhesion Blocking. Other applications not tested.
Working DilutionFlow Cytometry: 1:100-1:200 (0.5-1ug will label about 10e6 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (2550%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8.S.303
HostMouse
SourceRat
Concentration~1mg/ml
FormLiquid in PBS, 0.02% sodium azide
PurityPurified by affinity chromatography.
ImmunogenRat FTL-43 cells
SpecificityRecognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts and blocks binding of rat lymphocytes to purified rat ICAM-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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