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Anti -CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2)
Anti -CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation. | | | Catalog # | C2269 | | Hybridoma | Formed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse. | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Adhesion Blocking. Other applications not tested. | | Working Dilution | Flow Cytometry: 1:100-1:200 (0.5-1ug will label about 10e6 cells) | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (25–50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8.S.303 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Liquid in PBS, 0.02% sodium azide | | Purity | Purified by affinity chromatography. | | Immunogen | Rat FTL-43 cells | | Specificity | Recognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts and blocks binding of rat lymphocytes to purified rat ICAM-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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