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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD19

Anti -CD19

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC IF
CD19 (B-lymphocyte antigen CD19 or B- lymphocyte surface antigen B4) is a cell surface molecule that assembles with the antigen receptor of B lymphocytes (CD21, CD81 and CD225) and decreases the threshold for antigen receptor-dependent stimulation. Lymphocytes proliferate and differentiate in response to various antigens. CD19 is expressed on follicular dendritic cells and B cells. CD19 is present on B cells from the earliest recognizable B-lineage cells during development to B-cell blasts, but is lost on maturation to plasma cells. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated on thyrosine. Serine and threonine phosphorylation of CD19 occurs upon DNA damage. Defects in CD19 are a cause of hypogammaglobulinemia.
The CD19 antigen (90 kDa) is expressed from the earliest stages of B-progenitor development, on all peripheral B cells including germinal centre B cells and all B cell lines and B cell leukaemias tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturations to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
Catalog #C2269-51
ApplicationsSuitable for use in Indirect Immunofluorescence, Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Leukemia and lymphoma phenotyping and Studies of the CD19/21 complex in B cell activation and the control of B cell development. Other applications not tested.
Recommended DilutionImmunohistochemistry: Identifies normal and malignant CD19 positive B lymphocytes in cryostat frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1257
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHairy cell leukemia cells
SpecificityRecognizes human CD19.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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