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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD21 (CR2, C3d Receptor, EBV Receptor) (Biotin)

Anti -CD21 (CR2, C3d Receptor, EBV Receptor) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC
CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on granulocytes and monocytes.
Catalog #C2272-54B
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Acetone-fixed, frozen tissue sections only
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS NumberN/A
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K109
HostMouse
SourcePorcine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS containing sodium azide. Conjugated to Biotin
PurityPurified
ImmunogenCD21
SpecificityRecognizes porcine CD21, Mr 160kD. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer’s patches (ILPP); 93-97% in spleen; and 0% in thymus. Does not stain B cells in cord blood or spleen from porcine fetuses (105 days gestation).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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