| CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on granulocytes and monocytes. |
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| Catalog # | C2272-54B |
| Applications | Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1ug/10e6 cells |
| Immunohistochemistry: Acetone-fixed, frozen tissue sections only |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 5K109 |
| Host | Mouse |
| Source | Porcine |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS containing sodium azide. Conjugated to Biotin |
| Purity | Purified |
| Immunogen | CD21 |
| Specificity | Recognizes porcine CD21, Mr 160kD. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer’s patches (ILPP); 93-97% in spleen; and 0% in thymus. Does not stain B cells in cord blood or spleen from porcine fetuses (105 days gestation). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
