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You are here:Home » Antibodies » Abs to CD Markers 21-40 » Anti -CD21 (CR2, Complement Receptor 2, C3d Receptor, EBV Receptor) (PE)

Anti -CD21 (CR2, Complement Receptor 2, C3d Receptor, EBV Receptor) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC
CD21, an antigen with MW:150kD. CD21 is also known as CR2, complement receptor 2, Epstein Barr virus receptor (EBV-R) and C3d receptor. CD21 is expressed by certain B cells, follicular dendritic cells and a subset of immature thymocytes. CD21 serves as the receptor C3d, C3g and iC3band also as the receptor for Epstein Barr virus. CD21 is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells.1 Low density CD21 has also been reported on granulocytes and monocytes. This antibody stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer’s patches (ILPP); 93-97% in spleen; and 0% in thymus.2 The antibody does not stain B cells in cord blood or spleen from porcine fetuses (105 days gestation). 2
Catalog #C2272-54C
Applications• Flow cytometry 2
• Immunohistochemistry (acetone-fixed, frozen tissue sections only) 2
• Immunoprecipitation 2
Recommended DilutionFlow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K109
HostMouse
SourcePorcine
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, sodium azide and a stabilizing agent. Conjugated to PE.
PurityPurified
SpecificityRecognizes porcine CD21, Mr 160kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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   Download Specifications for Anti -CD21 (CR2, Complement Receptor 2, C3d Receptor, EBV Receptor) (PE)

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