| CD21 is a single-pass type 2 transmembrane protein that serves as the complement receptor for C3d and the Epstein-Barr virus. It is a member of a family of C3b/C4b binding proteins that regulate complement activation, comprise tandemly repeated 60-75 amino acid sequences, and whose genes map to band q32 on chromosome 1 (1). CD21 interacts with the p53 anti-oncoprotein, the p68 calcium binding protein and the nuclear p120 ribonucleoprotein to regulate their functions. Phosphorylation of CD21 inhibits its interaction with the nuclear p120 ribonucleoprotein (2). |
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| Catalog # | C2272-54E |
| Applications | Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:2000-1:5000 |
| Immunohistochemistry: 1:250-1:500 |
| Immunocytochemistry: 1:250-1:500 |
| Flow Cytometry: 1:20 |
| Immunoprecipitation: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9E195 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues on the C terminus of human CD21. |
| Specificity | Recognizes human CD21. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
