| Human CD21 is a receptor for complement fragments C3d, C3dg or ic3D and also EBV. CD21 is also a ligand for CD23 and plays a role in IgE synthesis. |
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| Catalog # | C2272-56B |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: frozen sections |
| Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Protocol | 1. 5x10e5 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of C2272-56B at 5ug/ml. |
| 2. Cells were washed twice and incubated with S7973-88P, Streptavidin/R-Phycoerythrin, after which they were washed three times fixed and analyzed using a FACS. |
| 3. Cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to I1904-78R2 IgG1/biotin Murine Negative Control at a similar concentration. |
| 4. Binding was 95% blocked when cells when preincubated 10 minutes with 20ul of 0.5mg/ml C2272-56A; CD21. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7H167 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human HFB-1 cell line. |
| Specificity | Recognizes human CD21 molecule of about 145kD and inhibits binding to CD23. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
