| CD21 is also known as CR2, C3d receptor and EBV receptor. Its epitope maps within 5-8 short consensus repeats (SCRs) of CD21 molecule. CD21 is expressed strongly on mature B cells, follicular dentritic cells, pharyngeal, cervical epithelial cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNa. CD21 is also known as Complement Receptor Type 2 (CR2) or Epstein-Barr virus (EBV) receptor. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. |
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| Catalog # | C2272-58 |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| Fusion Partners | Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line |
| Recommended Negative Controls | Mouse IgG2a Negative Control |
| Applications | Immunohistochemistry: (frozen)- 1:10-1:20, (paraffin)- 1:10-1:20 |
| Optimal working dilutions to be determined by researcher. |
| Immunohistochemistry | Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H1104 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Tissue Culture Supernatant lyophilized with 15mM sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. |
| Purity | Tissue culture supernatant. |
| Immunogen | Recombinant human CD21. |
| Specificity | Recognizes the human CD21 cell surface antigen, a 140 glycoprotein. CD21 is expressed by mature B lymphocytes. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
