| CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140kD protein. CD21 is a glycosylated type I transmembrane protein consisting on the extracellular face of a serie of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23-signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. |
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| Catalog # | C2272-58B |
| Applications | Can be used for Flow Cytometry and Western Blot. |
| Recommended Dilution | Flow Cytometry: 1:10 |
| Western Blot: 1:10 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG3 |
| Clone No | 6A499 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA. |
| Purity | Purified IgG |
| Specificity | Reacts with human CD21. Reacts with CD21 on B-cells and dendritic cells. This clone is a unique antibody which is also useful to recognize cells with low CD21 expression such as a subset of T cells, epithelial cells and astrocytes. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
