| CD21 is also known as CR2, C3d receptor and EBV receptor. Its epitope maps within 5-8 short consensus repeats (SCRs) of CD21 molecule. CD21 is expressed strongly on mature B cells, follicular dentritic cells, pharyngeal, cervical epithelial cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNa. CD21 is also known as Complement Receptor Type 2 (CR2) or Epstein-Barr virus (EBV) receptor. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. |
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| Catalog # | C2272-58D |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
| Applications | Immunoprecipitation |
| Flow Cytometry: Neat |
| Immunohistochemistry: (frozen)- 1:10-1:100 |
| Optimal working dilutions to be determined by researcher. |
| Flow Cytometry | Use 10ul of the suggested working dilution to label 10e6 cells or 100ul human whole blood. |
| Immunohistochemistry: Recommended positive control tissue: Lymph node. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1111 |
| Host | Mouse |
| Source | Human |
| Concentration | ~200 Tests/2ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by Ion Exchange chromatography. |
| Immunogen | Human IM9 cell line. |
| Specificity | CD21 is a 14kD cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
