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Specifications

Clone Host Grade Applications Monoclonal Rat Purified FC

CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression.1, 2 CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion.2-4 Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation.6-9
Catalog #	C2272-88AX
Applications	• Flow cytometry 2
	• Blocking of B cell homotypic adhesion 2
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5K249
Host	Rat
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS. No preservative added.
Purity	Purified
Specificity	Recongizes CD22 (Mr 150kD), a B lymphocyte adhesion molecule.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.