| CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression.1, 2 CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion.2-4 Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation.6-9 |
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| Catalog # | C2272-88AX |
| Applications | • Flow cytometry 2 |
| • Blocking of B cell homotypic adhesion 2 |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 2Q1605 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS. No preservative added. |
| Purity | Purified |
| Specificity | Recongizes CD22 (Mr 150kD), a B lymphocyte adhesion molecule. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
