| CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression.1, 2 CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion.2-4 Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation.6-9 |
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| Catalog # | C2272-88E |
| Applications | • Flow cytometry 2 |
| • Blocking of B cell homotypic adhesion 2 |
| Recommended Dilution | Flow Cytometry: 0.2ug/10e6 cells |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 2Q1605 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, sodium azide and a stabilizing agent. Conjugated to APC. |
| Purity | Purified |
| Specificity | Recongizes CD22 (Mr 150kD), a B lymphocyte adhesion molecule. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
