| CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas. |
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| Catalog # | C2272-90 |
| Applications | Immunohistochemistry: Paraffin. 1:20-1:40 |
| Optimal working dilutions to be determined by researcher. |
| Immunohistochemistry | Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose. |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| Fusion Partners | Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line |
| Recommended Positive Control Tissue | Tonsil |
| Recommended Negative Controls | Mouse IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | N/A |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1112 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Tissue Culture Supernatant lyophilized with 15mM sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. |
| Purity | Tissue culture supernatant. |
| Immunogen | Recombinant human CD22. |
| Specificity | Recognizes the human CD22 cell surface antigen, a 130-140kD glycoprotein. CD22 is expressed within the cytoplasm at an early stage of B cell differentiation, but is expressed at the membrane only on mature B lymphocytes. CD22 expression is lost during terminal differentiation to plasma cell stages. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
