| CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas. |
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| Catalog # | C2272-90D |
| Applications | Flow Cytometry: Neat. Use 5-10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher. |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
| Recommended Secondary Reagents | F(ab')2 Goat anti-rat IgG:RPE |
| Recommended Negative Controls | Rat IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1116 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Specificity | Recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
