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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified FC

CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression. CD22 reacts with most B cell leukemias including Hairy Cell Leukemia (HCL) and B cell lymphomas.
Catalog #	C2272-90F
Applications	Flow Cytometry: Neat. Use 5-10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher.
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Recommended Secondary Reagents	F(ab')2 Goat anti-rat IgG:RPE
Recommended Negative Controls	Rat IgG1 Negative Control
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H1116
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS.
Purity	Purified by Protein G affinity chromatography.
Specificity	Recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.