Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC IF

CD22 antigen is expressed in the cytoplasm of pro-B cells and pre-B cells, and on the surface of mature B lymphocytes. After activation, the level of CD22 antigen expression is increased, and is lost during the terminal stage of B cell differentiation. Most B cell leukemias and lymphomas also express CD22 antigen which is absent from T cells, monocytes and granulocytes. Detecting the cytoplasmic CD22 antigen is valuable for diagnosing precisely early B-ALL. Also CD22 is one of the important markers to diagnose hairy-cell leukemia (HCL). CD22 antigen is an adhesion molecule and may augment the signal of B cell activation. The ligands for CD22 are CD45RO antigen on T cells and CD75 antigen on B cells participating in the homotypic interaction of T and B cells.
Catalog #	C2272-92
Applications	Suitable for use in indirect Immunofluorescence, Flow Cytometry and Immunohistochemical. Other applications not tested.
Recomended Dilutions	Flow Cytometry: 0.5ug labels10e6 cells
	Immunohistochemistry: Acetone fixed frozen
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F25
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	CD22
Specificity	Recognizes a 130-140kD type I transmembrane glycoprotein which is a B cell restricted antigen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.