| CD22 antigen is expressed in the cytoplasm of pro-B cells and pre-B cells, and on the surface of mature B lymphocytes. After activation, the level of CD22 antigen expression is increased, and is lost during the terminal stage of B cell differentiation. Most B cell leukemias and lymphomas also express CD22 antigen which is absent from T cells, monocytes and granulocytes. Detecting the cytoplasmic CD22 antigen is valuable for diagnosing precisely early B-ALL. Also CD22 is one of the important markers to diagnose hairy-cell leukemia (HCL). CD22 antigen is an adhesion molecule and may augment the signal of B cell activation. The ligands for CD22 are CD45RO antigen on T cells and CD75 antigen on B cells participating in the homotypic interaction of T and B cells. |
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| Catalog # | C2272-92 |
| Applications | Suitable for use in indirect Immunofluorescence, Flow Cytometry and Immunohistochemical. Other applications not tested. |
| Recomended Dilutions | Flow Cytometry: 0.5ug labels10e6 cells |
| Immunohistochemistry: Acetone fixed frozen |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5F25 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | CD22 |
| Specificity | Recognizes a 130-140kD type I transmembrane glycoprotein which is a B cell restricted antigen. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
