| CD22, a negative regulator of B cell signaling, belongs to the sialoadhesin family of receptors that preferentially binds to 2-6-linked sialic acid on glycoproteins (1-2). CD22 is known to interact with the B cell antigen receptor (BCR) (3) and the tyrosine phosphatase SHP1. Tyrosine phosphorylation of CD22 leads to the recruitment of multiple intracellular Src homology 2 (SH2) domain-containing effector molecules, including phospholipase C and Syk, which are important for triggering Ca2+ influx, as well as PI 3-kinase and Grb2 (4-5). |
|
| Catalog # | C2272-98B |
| Applications | Suitable for use inWestern Blot. Other applications not tested. |
| Recommended Dilution | Western Blotting: 1:1,000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 6k104 |
| Host | Rabbit |
| Source | Human |
| Form | Supplied as a liquid |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues near the N-term of human CD22 |
| Specificity | Recognizes human CD22 |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
