| The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes. The CD22 antigen is expressed on > 90% of peripheral blood B-cells. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia. This antigen is not present on T-cell, monocytes or macrophages. |
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| Catalog # | C2272-98F |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA (indirect and sandwich): 0.5-2.0ug/ml |
| Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant hCD22 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| Country of Origin: |
| USA |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Highly pure (> 98%) recombinant human soluble CD22 |
| Specificity | Recognizes human CD22. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
