| The CD23 antigen is the low affinity IgE Fc receptor, which is a 49kD protein with 38 and 28kD fragments.1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets.1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.2-6 CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.6 |
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| Catalog # | C2273-91D |
| Applications | • Identification and enumeration of CD23+ cells by flow cytometry 2, 5 |
| • Differentiation of B cell subpopulations by cell sorting 7 |
| • Immunoprecipitation of CD23 1 |
| Recommended Dilution | Flow Cytometry: 0.1ug/10e6 cells |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 2Q1609 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, sodium azide and a stabilizing agent. Conjugated to PE. |
| Purity | Purified |
| Specificity | Recognizes CD23 low affinity IgE Fc receptor. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
