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Specifications

Clone Host Grade Applications Monoclonal Rat Purified IP FC

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49kD protein with 38 and 28kD fragments.1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets.1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.2-6 CD23 has been described as a “membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.6
Catalog #	C2273-91D
Applications	• Identification and enumeration of CD23+ cells by flow cytometry 2, 5
	• Differentiation of B cell subpopulations by cell sorting 7
	• Immunoprecipitation of CD23 1
Recommended Dilution	Flow Cytometry: 0.1ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	2Q1609
Host	Rat
Source	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, sodium azide and a stabilizing agent. Conjugated to PE.
Purity	Purified
Specificity	Recognizes CD23 low affinity IgE Fc receptor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.