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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)

Anti -CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor
Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4).
Catalog #C2275-83A
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Frozen sections
Flow Cytometry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM).
2. Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of aC2275-83A at 5ug/ml.
3. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No7H136
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
PurityPurified by Protein A affinity chromatography.
ImmunogenStimulated PBMC
SpecificityRecognizes epitope B of the human IL-2 alpha receptor of about 55kD. Does not block IL-2 binding to its receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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