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Anti -CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)
Anti -CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor
Receptor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4). | | | Catalog # | C2275-83A | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: Frozen sections | | Flow Cytometry: 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Flow Cytometry Protocol | 1. Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). | | 2. Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of aC2275-83A at 5ug/ml. | | 3. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. | | 4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 7H136 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Stimulated PBMC | | Specificity | Recognizes epitope B of the human IL-2 alpha receptor of about 55kD. Does not block IL-2 binding to its receptor. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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