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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2)

Anti -CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human
homolog of Drosophila Double Parked, RIS2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Geminin is a protein that inhibits DNA replication by preventing the loading of MCM complex on chromatin. Human CDT1 is a 65kDa protein that contains 546 amino acids and coimmunoprecipitates with Geminin. Inhibition of DNA replication by Geminin in cell-free cDNA replication extracts can be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas Geminin is present in S and G2 phases.
Catalog #C2300-02
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2500
Immunoprecipitation: 2-5ug/mg lysate
Immunohistochemistry (paraffin): 1:100-1:500. Requires heating in citrate buffer, pH 6 for antigen retrieval.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, pH7-8, containing 0.09% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide representing the C-terminus of the human Chromatin Liscensing & DNA Factor 1 protein (residues 500-546) according to NP_112190.1.
SpecificityRecognizes human CDT1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2


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