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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD31 (PECAM-1, Platelet gpIIa Molecule)

Anti -CD31 (PECAM-1, Platelet gpIIa Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
PECAM-1 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. Reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with a subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes. PECAM-1 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1. The monoclonal antibody can be used, in combination with the monoclonal antibody, for
identification of different stages of myeloid progenitor cell development.
Catalog #C2383-02H
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunohistochemistry (frozen or paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G146
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified
ImmunogenMouse macrophage cell lines.
SpecificityRecognizes an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. The mouse hematopoietic progenitor cell antigen is Platelet-Endothelial Cell Adhesion Molecule (PECAM)-1 (CD31) of 113kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD31


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