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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD31 (PECAM-1, Platelet gpIIa Molecule)

Anti -CD31 (PECAM-1, Platelet gpIIa Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC IF
CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable in the study of benign and malignanat vaascular tumors. Stainiing for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Catalog #C2383-10A
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10-1:100. Use 10ul of diluted antibody to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:10-1:100
Immunohistochemistry (paraffin): Staining of endothelium may be enhanced on FFPE tissue by pre-treatment with 0.2M boric acid, pH 7.0 ( see Wilson et al.)
Functional Studies: Recommend the use of C2383-03G.
Optimal working dilutions to be determined by researcher.
HybridomaSp2/myeloma cells with spleen cells from Balb/c mice.
Recommended Negative ControlI1904-78Y: IgG1 Mouse Negative Control (rat tissues only).
Recommended Secondary ReagentsI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H1217
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenActivated, Lewis rat derived microglial cells.
SpecificityRecognizes rat PECAM-1. Partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. Species Crossreactivity: Does not crossreact with human tissues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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