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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell
Antigen CD34, HPCA1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells. This antibody is of value in the study of benign and malignant vascular tumors as well as characterization of leukemias. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Catalog #C2386-10
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:200 for 20-60 min at RT. May require special pretreatment for histochemical staining of formalin-fixed tissues by boiling tissues in10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlPlacenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F85
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2386-10X
PurityPurified by Protein G affinity chromatography.
ImmunogenDetergent solubilized vesicular suspension prepared from a perfusate of human term placenta. MW of Antigen: 105-120kD
EpitopeType II epitope. Cellular Localization: Cell membrane / cytoplasmic.
SpecificityRecognizes human CD34. Also reacts with vascular endothelial cells in normal tissues and in benign and malignant proliferations. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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