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You are here:Home » Antibodies » Antibodies-CD Markers » Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell
Antigen CD34, HPCA1)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
CD34 is a 110-120kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352aa protein with a 257 aa extracellular domain (ECD) (aa35-291) and 74aa cytoplasmic tail (aa313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.
Catalog #C2386-12A
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 5-15ug/ml stains perfusion fixed frozen sections of rat liver.
Western Blot: 0.1ug/ml stains recombinant rat CD34.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa37-293 from rat CD34 expressed in NS0 cells (NP_001100672). Species Sequence Homology: mouse, 83%; human, 57%
SpecificityRecognizes rat CD34. Shows <1% crossreactivity with recombinant porcine CD34.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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