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Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)
Anti -CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell
Antigen CD34, HPCA1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| CD34 is a 110-120kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352aa protein with a 257 aa extracellular domain (ECD) (aa35-291) and 74aa cytoplasmic tail (aa313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively. | | | Catalog # | C2386-12A | | Applications | Suitable for use in Immunohistochemistry, Western Blot and Direct ELISA. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry: 5-15ug/ml stains perfusion fixed frozen sections of rat liver. | | Western Blot: 0.1ug/ml stains recombinant rat CD34. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Rat | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant protein corresponding to aa37-293 from rat CD34 expressed in NS0 cells (NP_001100672). | | Specificity | Recognizes rat CD34. Shows <1% crossreactivity with recombinant porcine CD34. Species Sequence Homology: mouse, 83%; human, 57% | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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