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You are here:Home » Growth Factors, Cytokines » Growth Factors-TRAP » CD40 Ligand, Soluble, Recombinant, Human (CD40L, CD40-L, hCD40L, CD40LG, CD154, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen Gp39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNFSF5)

CD40 Ligand, Soluble, Recombinant, Human (CD40L, CD40-L, hCD40L, CD40LG, CD154, gp39,
HIGM1, IGM, IMD3, T-BAM, T-cell Antigen Gp39, TNF-related Activation Protein, TRAP, Tumor
Necrosis Factor Ligand Superfamily Member 5, TNFSF5)

Pricing

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Specifications

Catalog #C2392-31H
SourceRecombinant corresponding to 149aa of human soluble CD40(CD154) Ligand/TRAP expressed in E.coli is a non-glycosylated, polypeptide chain.
Molecular Weight~16.3kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10nug/ml.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of soluble CD40L as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~16.3kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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