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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD41 (IIB-IIIA)

Anti -CD41 (IIB-IIIA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH IC FC IF
The antibody precipitates a complex of molecular weights 122kD and 112kD when reduced and 87kD and 140kD when not reduced (1). The IIb-IIIa complex is a member of the integrin superfamily of adhesive receptors and mediates platelet-platelet aggregation. This complex recognizes the Arg-Gly-Asp (RGD) tripeptide sequence in adhesive glycoproteins such as fibronectin, fibrinogen and von Willebrand Factor thereby facilitating linkage between adjacent platelets. This antibody is useful in biochemical studies of the Glycoprotein IIb-IIIa complex and the enumeration of platelets. The antibody specifically inhibits ADP and collagen induced aggregation and release of ATP, and ADP induced platelet fibrinogen binding1. The antibody may also be used for immunoperoxidase staining of formalin fixed cell suspensions and frozen tissue sections.
Catalog #C2394-03A
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:25 Dilute with isotonic buffer. Use 50ul per 1x10e6 platelets in 100ul of buffer.
Indirect Immunofluorescence: 1:25 Dilute with isotonic buffer. Use 50ul per 1x10e6 platelets in 100ul of buffer.
Indirect Immunoperoxidase: 1:25
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q948
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 0.2% BSA.
PurityPurified >95% by SDS-PAGE
ImmunogenHuman Platelet membranes
SpecificityRecognizes the human Glycoprotein IIb-IIIa complex on the surface of platelets and megakaryocytes. It is not reactive with T and B cells or with other haemopoietic cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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