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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)

Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B FC
GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD.
Catalog #C2395-20B
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhGPIb-alpha is ~1ng/well.
Western Blot: 1-2ug/ml detection limit for rhGPIb-alpha is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H211
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rhGPIb-alpha. (aa 17 - 505)
SpecificityRecognizes human GPIb-alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGlycocalicin


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