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Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)
Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B FC |
|
| GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD. | | | Catalog # | C2395-20B | | Applications | Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells. | | Direct ELISA: 0.5-1.0ug/ml detection limit for rhGPIb-alpha is ~1ng/well. | | Western Blot: 1-2ug/ml detection limit for rhGPIb-alpha is ~ 5ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8H211 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived rhGPIb-alpha. (aa 17 - 505) | | Specificity | Recognizes human GPIb-alpha. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Glycocalicin |
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