Anti -CD44S (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Purified |
E B IP IH FC IF RIA |
|
| CD44 (H-CAM, Homing Cell Adhesion Molecule) is a 85-90kD transmembrane protein. The human CD44 gene contains 19 exons; 1-5 and 15-19 are expressed by wild-type CD44, also known as the standard hemopoietic form (CD44s). | | | Catalog # | C2398-85 | | Applications | Suitable for use in ELISA, RIA, Flow Cytometry, Immunoflourescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | ELISA: 1:2000 | | RIA: 1:5000 | | Flow Cytometry: 1:1000 | | Immunoflourescence: 1:200 | | Immunohistochemistry (Paraffin): 1:250 Antigen retrieval is usually not needed. | | Immunoprecipitation: 1:200 | | Western Blot: 1:1000. Non-reduced only. ~90kD band detected; Blocking PBS/5% milk protein. Overnight incubation. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 2Q1374 | | Host | Rat | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.5, 0.01% sodium azide. | | Purity | Purified | | Immunogen | Purified human blood lymphocyte CD44 | | Specificity | Recognizes human CD44 (85-95kD) gp90 Hermes and mouse gp85 (Pgp-1). Reacts with the standard CD44 isoform that lacks exon sequences v1-v10. Species Crossreactivity: rabbit, porcine, mouse, rat, canine, bovine cells/tissue | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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