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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD44S (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

Anti -CD44S (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes
Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B IP IH FC IF RIA
CD44 (H-CAM, Homing Cell Adhesion Molecule) is a 85-90kD transmembrane protein. The human CD44 gene contains 19 exons; 1-5 and 15-19 are expressed by wild-type CD44, also known as the standard hemopoietic form (CD44s).
Catalog #C2398-85
ApplicationsSuitable for use in ELISA, RIA, Flow Cytometry, Immunoflourescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA: 1:2000
RIA: 1:5000
Flow Cytometry: 1:1000
Immunoflourescence: 1:200
Immunohistochemistry (Paraffin): 1:250 Antigen retrieval is usually not needed.
Immunoprecipitation: 1:200
Western Blot: 1:1000. Non-reduced only. ~90kD band detected; Blocking PBS/5% milk protein. Overnight incubation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No2Q1374
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.01% sodium azide.
PurityPurified
ImmunogenPurified human blood lymphocyte CD44
SpecificityRecognizes human CD44 (85-95kD) gp90 Hermes and mouse gp85 (Pgp-1). Reacts with the standard CD44 isoform that lacks exon sequences v1-v10. Species Crossreactivity: rabbit, porcine, mouse, rat, canine, bovine cells/tissue
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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