Anti -CD44V10 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1,
Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B IP IH FC IF RIA |
|
| Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1–15 and exons 15–19. The other exons (e.g. 6–14) are also spliced in a variety of different cells. For example, epithelial cells contain exons 12–14 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis. | | | Catalog # | C2398-86 | | Applications | Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, RIA and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunofluorescence: 1:200 | | Flow Cytometry: 1:1000 | | ELISA: 1:2000 | | Western Blot: 1:10,000 | | Immunohistochemistry (Paraffin): 1:250 | | Immunoprecipitation: 1:200 | | RIA: 1:5000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.01% sodium azide. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to human CD44v10. | | Specificity | Recognizes human CD44v10 (~116kD). Species Crossreactivity: rabbit, porcine, mouse, rat, canine and bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
