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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » CD45, aa584-1256, Protein Tyrosine Phosphatase, Catalytic Domain, N-terminal His-Tag, Recombinant, Human (Leukocyte Common Antigen, LCA, Ly-5 T200)

CD45, aa584-1256, Protein Tyrosine Phosphatase, Catalytic Domain, N-terminal His-Tag,
Recombinant, Human (Leukocyte Common Antigen, LCA, Ly-5 T200)

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Specifications

PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
Catalog #C2399-02B
Protein tyrosine phosphatase CD45, also known as PTPRC (Accession# NM_080922), catalytic domain, aa632-1304 (end) with N-terminal His-tag, MW=81kD, expressed in a Baculovirus Sf9 expression system.
ApplicationSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Specific ActivityAs Reported
Assay buffer20mM HEPES, pH 7.2, 100mM sodium chloride, 2mM EDTA, 1mM DTT, 1mM PNPP, 20 min at RT. Absorbance was measured at 405nm. Specific activity was calculated based on pnp=18,000M-1cm-1
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~81kD
SourceSf9 insect cells
Purity90% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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