CD45, aa584-1256, Protein Tyrosine Phosphatase, Catalytic Domain, N-terminal His Tag, Recombinant, Human (Leukocyte Common Antigen, LCA, Ly-5 T200)
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| Catalytic domain, (a.a. 584-1256) with N-terminal His tag, MW=84.5kD, expressed in a Sf9 baculovirus expression system. | | | Catalog # | C2399-02B | | Stability | >6 months at –80 °C | | Purity | >90% by SDS-PAGE. | | Specific Activity | 25 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C | | Assay buffer | 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP. | | Application | useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. | | | | Source | Recombinant, Human from sf9 insect cells | | Form | 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 5 mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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