Anti -CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5,
Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C
Polypeptide, PTPRC, T200, T200 Glycoprotein)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IH FC |
|
| CD45 or Leukocyte Common Antigen (LCA/T200) family is comprised of at least four isoforms (CD45RA, CD45RB, CD45RC, and CD45RO) of membrane glycoproteins with MW of 180-220kD. It consists of a large, heavily glycosylated, amino-terminal extracellular domain of 400-550 amino acids, a single transmembrane region and a large carboxy-terminal cytoplasmic domain of 700 amino acids. CD45/LCA is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of CD45 proteins has phosphatase activity and is involved in regulation of transmembrane signals, functioning as a leukocyte membrane receptor capable of transducing signals via its phosphotyrosine phosphatase domains. | | | Catalog # | C2399-07G2 | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul | | Immunohistochemistry (Frozen): 1:500-1:1000 | | Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA, pH8.0 is recommmeded. | | Optimal dilutions to be determined by the researcher. | | Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. | | Recommended Positive Control | Human Tonsil | | Recommended Negative Control | I1904-83P1: IgG2a Murine Negative Control | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 10B1611 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Human T lymphocytes | | Specificity | Recognizes human CD45. Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. | | Species crossreactivity | Equine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
|
Questions or comments about this product? Inquire here. |
|
|
Download Specifications for Anti -CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) |
External Links
|
