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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Azide Free)

Anti -CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 while those recognizing only individual isoforms are termed CD45RA or CD45RO etc.
Catalog #C2399-08V
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunopreciptiation. Other applications have not been tested.
Recommended DilutionsFlow Cytometry: 1:100-1:200. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose.
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsI1904-65V: IgG, F(abí)2 (FITC) Pab RbxMo
I1904-66A: IgG, F(abí)2, X-Adsorbed (FITC) Pab RbxMo
I1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
F(ab')2 rabbit anti mouse IgG: RPE
HybridomaNS-1 myeloma cells with spleen cells from Balb/c mice.
IHC Positive ControlHuman tonsil
Negative ControlMouse IgG2a Negative Control, azide free
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H1378
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives added.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman T lymphocytes
SpecificityRecognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher levels of expression on lymphocytes than on granulocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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