| CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 while those recognizing only individual isoforms are termed CD45RA or CD45RO etc. |
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| Catalog # | C2399-08V |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Immunopreciptiation. Other applications have not been tested. |
| Recommended Dilutions | Flow Cytometry: 1:100-1:200. Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry (Frozen and paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Reagents | I1904-65V: IgG, F(ab’)2 (FITC) Pab RbxMo |
| I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab RbxMo |
| I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| F(ab')2 rabbit anti mouse IgG: RPE |
| Hybridoma | NS-1 myeloma cells with spleen cells from Balb/c mice. |
| IHC Positive Control | Human tonsil |
| Negative Control | Mouse IgG2a Negative Control, azide free |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H1378 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. No preservatives added. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human T lymphocytes |
| Specificity | Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher levels of expression on lymphocytes than on granulocytes. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
