CD45

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG2a

Clone Number

9F609

Grade

Affinity Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD45 is a transmembrane tyrosine phosphatase, expressed on nucleated hematopoitic cells, which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing. CD45 functions as a leukocyte membrane receptor signal transduction. It plays an essential role in the regulation of leukocyte activation, development, and function.

Applications

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Human lymphocytes

Form

Supplied as a liquid in PBS, pH 7.2.

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes all isoforms of human CD45 (LCA).

References

Mustelin, T. et al. (2004) Mol. Immunol. 41,687-700 ; Trowbridge, I.S. et. al. (1994) Annu. Rev. Immunol. 12,85-116 ; Justement, L.B. et. al. (1991) Science 252,1839-1842.