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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD45RO (Biotin)

Anti -CD45RO (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive in ltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes. When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore, anti-CD45RO may be a marker for memory T-cells.
Catalog #C2400-67
ApplicationsSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Formalin-fixed, paraffin-embedded tissues; frozen sections; cell suspensions.
Flow Cytometry: 1ug to stain 1x10e6 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.BB.437
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenIL-2 dependent T-cell line CA1
SpecificityRecognizes the low MW (Mr 180kD) isoform of the human leukocyte common antigen (LCA) which is part of a family of protein tryosine phosphatases.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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