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Anti -CD45RO (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive in ltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes. When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore, anti-CD45RO may be a marker for memory T-cells. | | | Catalog # | C2400-67 | | Applications | Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications have not been tested. | | Recommended Dilutions | Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues; frozen sections; cell suspensions. | | Flow Cytometry: 1ug to stain 1x10e6 cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 1.BB.437 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | IL-2 dependent T-cell line CA1 | | Specificity | Recognizes the low MW (Mr 180kD) isoform of the human leukocyte common antigen (LCA) which is part of a family of protein tryosine phosphatases. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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