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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP FC IF

CD47 antigen, also known as integrin associated-protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
Catalog #	C2402-02
MW of Antigen	47-52kD (reduced) and 45-50.5, 110kD (non-reduced)
Epitope	Ig domain
Applications and Recommended Dilutions	Blocks binding of SIRPa4,5
	Inhibits in assays in which CD47-integrin association is required6,7,8
	Flow Cytometry
	Immunofluorescence (Methanol fixed only)
	Immunoprecipitation (Native only, Use Protein G) (Ab 2ug/mg protein lysate) The optimal dilution for a specific application should be determined by the investigator.
Positive Control	Tonsil
Cellular Localization	Cell membrane
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3F95
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2402-02X
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact CD47 purified from placenta.
Specificity	Blocks binding of SIRP alpha and in assays of integrin association. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.