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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD47

Anti -CD47

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC IF
CD47 antigen, also known as integrin associated-protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
Catalog #C2402-02
MW of Antigen 47-52kD (reduced) and 45-50.5, 110kD (non-reduced)
Epitope Ig domain
Applications and Recommended DilutionsBlocks binding of SIRPa4,5
Inhibits in assays in which CD47-integrin association is required6,7,8
Flow Cytometry
Immunofluorescence (Methanol fixed only)
Immunoprecipitation (Native only, Use Protein G) (Ab 2ug/mg protein lysate) The optimal dilution for a specific application should be determined by the investigator.
Positive Control Tonsil
Cellular Localization Cell membrane
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F95
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2402-02X
PurityPurified by Protein G affinity chromatography.
ImmunogenIntact CD47 purified from placenta.
SpecificityBlocks binding of SIRP alpha and in assays of integrin association. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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