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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified B IH FC

Catalog #	C2402-03F
Applications	Suitable for use in Flow cytometry, Western blot (non-reducing) and Immunohistochemistry (). Other applications have not been tested.
Recommended Dilutions	Western Blot: Non-reducing
	Immunohistochemistry: frozen sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	8J217
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
Purity	Purified by CHT chromatography and precipitation methods.>95% pure (SDS-PAGE).
Immunogen	COS-7 (African green monkey) cells
Specificity	Recognizes human CD47 (Integrin Associated Protein), a 50-55kD membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. Reacts with pig and non-human primates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.