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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD47

Anti -CD47

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
Catalog #C2402-04
ApplicationsSuitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:100 Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher.
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Recommended Secondary ReagentsIgG Goat anti mouse IgG:FITC (rat adsorbed)
IgG Goat anti mouse IgG:RPE (rat adsorbed)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A296
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat thymocytes.
SpecificityRecognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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