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You are here:Home » Antibodies » Abs to Integrin » Anti -CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)

Anti -CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen
B)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH FC IF
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines.
Catalog #C2404-07B
CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets.
ApplicationsSuitable for use in Indirect Immunofluorescence, FACS, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Molecular Weight 130,170kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1008
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 20mM Tris, 150mM sodium chloride, pH 8.0, 10mg/ml BSA, 0.001% merthiolate.
PurityPurified by ammonium sulfate precipitation and ion exchange chromatography.
ImmunogenHuman T lymphocytes
SpecificityDirected against the a2 human integrin (CD49b antigen, GPIa or VLA-2 alpha chain). Reacts with platelets, long-term cultivated T lymphocytes and activated T lymphocytes. Reacts with thymocytes, epithelial cells of a variety of tissues, peripheral nerves, fibroblasts, osteoclasts, glomerular mesangium and most non-hematopoietic adherent cell lines.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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