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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B) (FITC)

Anti -CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen
B) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and functions as an adhesion molecule.
Catalog #C2404-07K
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50. Use 80ul per test.
Optimal dilutions to be determined by the researcher.
FACS Protocol1. Five x 10e5 cultured UM-SCC (squamous cell carcinoma) cells were harvested by trypsinization, washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (To block nonspecific binding). After which, they were incubated 45 minutes on ice with 80ul of C2404-07K at a 1:50 dilution.
2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration.
4. Binding was 91% blocked when pre-incubated 10 minutes with 20ul of 0.5ug/ml C2404-07H.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No7H189
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Fluorescein/IgG Ratio: 6.2
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cultured keratinocytes
SpecificityRecognizes the human alpha 2 integrin subunit (CD49b).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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