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Anti -CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)
Anti -CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IH FC |
|
| Catalog # | C2404-08D | | Applications | Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. | | Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). | | Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. | | Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 2Q995 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Human squamous cell carcinoma cells (SCC9). | | Specificity | Recognized human a3 integrin (CD49c). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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