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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IP

CD49e is a type I membrane protein that associates non-covalently with integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.
Catalog #	C2405-02A
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:500 for detection of whole cell lysate.
	Immunoprecipitation: 3ul per 10e6 cell equivalent
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Serum
Immunogen	Synthetic peptide corresponding to the C-terminal cytoplasmic domain of human alpha 5 integrin.
Specificity	Recognizes human integrin alpha 5, as confirmed by western blot and immunoprecipitation. Not reactive with other integrins. Species Crossreactivity: mouse, rat and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.