| CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration. |
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| Catalog # | C2405-02D |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and RIA. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:500-1:2000 |
| Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. Use denatured but non-reduced samples to detect full length product. ECL; BSA blocking suggested. |
| Immunoprecipitation: Use 5ul antibody for 5x10e6 cells. |
| Immunohistochemistry (Acetone fixed tissues): 1:1000 |
| Immunocytochemistry: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Control | Mouse 3T3 fibroblasts, skin (basement membrane) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in buffer with 0.05% sodium azide. |
| Purity | Serum |
| Immunogen | Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit. |
| Specificity | Recognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. Species Crossreactivity: chicken, mouse, rat and hamster. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
