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You are here:Home » Antibodies » Abs to Integrin » Anti -CD49e (Integrin alpha5)

Anti -CD49e (Integrin alpha5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IC IF RIA
CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.
Catalog #C2405-02D
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and RIA. Other applications not tested.
Recommended DilutionsELISA: 1:500-1:2000
Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. Use denatured but non-reduced samples to detect full length product. ECL; BSA blocking suggested.
Immunoprecipitation: Use 5ul antibody for 5x10e6 cells.
Immunohistochemistry (Acetone fixed tissues): 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
ControlMouse 3T3 fibroblasts, skin (basement membrane)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit (NP_002196).
SpecificityRecognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. Species Crossreactivity: chicken, mouse, rat and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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