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C2405-02D Rabbit Anti-CD49e (Integrin alpha5) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P08648
Isotype
IgG
Grade
Serum
Applications
E IC IHC IP WB
Crossreactivity
Ch Hm Hu Mo Rt
Accession #
NP_002196
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:500-1:2000 Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. HUVEC lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C2405-02D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: Use 5ul antibody for 5x10e6 cells. Immunohistochemistry (Acetone fixed tissues): 1:1000 Immunocytochemistry: 1:1000 Optimal dilutions to be determined by the researcher.
Control
Mouse 3T3 fibroblasts, skin (basement membrane)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. |Species Crossreactivity: chicken, mouse, rat and hamster.
References
1. Ann. Rev. Biochem. 57: 375 (1988). 2. Science 238: 491 (1987). 3. Cell 48: 549–554 (1987). 4. Cell 56: 281–290 (1989). 5. Exper. Cell Res. 189: 100–108 (1990). (immunoprecipitation protocol) 6. Research in Veterinary Science 57: 358–364 (1994). 7. Mol. Biol. of the Cell 10: 785–798 (1999). 8. Cancer Research 60: 309–320 (2000). 9. Sherry D.M. & Proske, P., Graefe’s Arch. Clin. Exp. Ophthalmol. 239: 278–287 (2001). 10. Garcia, P., et al., Anim. Reprod. Sci. 80(1-2): 91-100 (2004). 11. Kitsiou, P.V., et al., Am. J. Physiol. Renal Physiol. 284: F671-F679 (2003). 12. Prouix, S., et al., Mole Vision 9: 473-481 (2003). 13. Segat, D., et al., J. Biol. Chem. 277(35): 31,612-31,622 (2002). 14. Gao, B., et al., Amer. J. Physiol. Cell Physiol. 283(4): C1196-C1205 (2002).
USBio References
No references available
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