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Specifications

Catalog #	C2405-10
TMB (3,3’,5,5’-Tetramethyl-benzidine) is a non-carcinogenic chromogen, which is widely used in ELISA where Horseradish Peroxidase is applied. The product is comprised of two components	TMB Solution A and TMB Solution B.
Stability	Avoid direct light
Procedure	Mix one part TMB Solution A with one part TMB Solution B together in a clean container. Mix completely, 15 minutes prior to use. This solution can now be applied to the wells as substrate.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source	Molecular Biology Grade
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.