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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD51 (Integrin alphaV, Vitronectin alpha Subunit Receptor, VNR)

Anti -CD51 (Integrin alphaV, Vitronectin alpha Subunit Receptor, VNR)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH RIA
The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.
Catalog #C2405-15F
ApplicationsSuitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsELISA: 1:1000
RIA: 1:1000
Western Blot: 1:500 Recognizes a single band of 150kD when denatured, but not reduced. Under reducing conditions recognizes two major bands of 25 and 27kD, and to a lesser extent the full length 150kD band. This pattern is consistent with the fact that mature alpha V is composed of a disulfide bonded heavy (125kD) and a light (25kD) chains derived from a proteolytically cleaved precursor.
Immunoprecipitation: 5ul of antibody is sufficient to precipitate alpha V/beta 1 from 5x10e6 cells.
Immunohistochemistry: 1:1000 Acetone fixed tissue only.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to the COOH terminal sequence (cytoplasmic domain), aa1022-1034 of human alpha V integrin subunit (NM_002210.2)
SpecificityRecognizes human CD51 (Integrin alpha V). The epitope recognized is on the cytoplasmic domain of the protein, and it is thus intracellular. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4 or alpha 6 integrin subunits. Species Crossreactivity: goat, equine, porcine, sheep, chicken, hamster, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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