Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC IF

CD65, known as CD65s (VIM-2 antigen), a leukocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.
Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (2).
Catalog #	C2405-21B
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Functional Assays. Other applications not tested.
Recommended Dilution	Flow Cytometry: (neat)1:200. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
	Immunohistochemistry (frozen): 1:20
	Optimal dilution to be determined by researcher.
Endotoxin Level	<0.1EU/ug
Positive Control	Human tonsil
Hybridoma	Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	9A146
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4.
Purity	Purified by ammonium sulfate precipitation.
Immunogen	THP1 (human acute monocytic leukaemia cells)
Specificity	Recognizes the sialylated form of human CD65.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.