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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CD65s, Low Endotoxin

Anti -CD65s, Low Endotoxin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH FC IF
CD65, known as CD65s (VIM-2 antigen), a leukocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.
Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (2).
Catalog #C2405-21B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Functional Assays. Other applications not tested.
Recommended DilutionFlow Cytometry: (neat)1:200. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20
Optimal dilution to be determined by researcher.
Endotoxin Level <0.1EU/ug
Positive ControlHuman tonsil
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No9A146
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4.
PurityPurified by ammonium sulfate precipitation.
ImmunogenTHP1 (human acute monocytic leukaemia cells)
SpecificityRecognizes the sialylated form of human CD65.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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