| CD55 is a cell surface antigen. It is a GPI linked molecule also known as Decay Accelerating Factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak. |
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| Catalog # | C2410-02 |
| Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: 1:5-1:12.5. Use 20ul to label 10e6 cells in 100ul. |
| Immunohistochemistry (frozen): 1:50-1:500 |
| Optimal dilutions to be determined by researcher. |
| Note | Use of this reagent to stain erythrocytes is not recommended. |
| Recommended Secondary Reagents | (FC) |
| I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab Rb xMo |
| I1903-13A: IgG, F(ab’)2, X-Adsorbed (PE) Pab Gt xMo |
| Recommended Secondary Reagents | (IHC) |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo |
| Recommended Positive Control Tissue | (IHC) |
| Human Tonsil |
| Recommended Negative Controls | (IHC) |
| I1904-78R: IgG1 Negative Control Mab Mo x |
| Source | Tissue culture |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1563 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | K562 cells |
| Specificity | Recognizes the human CD55 cell surface antigen. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
