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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD62E (LECAM, E-Selectin, ELAM1, Endothelial Leukocyte Adhesion Molecule)

Anti -CD62E (LECAM, E-Selectin, ELAM1, Endothelial Leukocyte Adhesion Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP IH FC
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion
receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that
is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Catalog #C2414-94B
ApplicationsSuitable for use in Western Blot, ELISA, Immunoprecipitation,
Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Frozen sections
Flow Cytometry: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4A24
HostRat
SourceMouse
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
PurityPurified by Protein G affinity chromatography.
ImmunogenCD62E (LECAM, E-Selectin, ELAM1, Endothelial Leukocyte Adhesion Molecule)
SpecificityRecognizes a conformational epitope in the CR2 region of mouse CD62E. Partially inhibits leukocyte adhesion to mouse activated endothelial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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